Quantifying molecular colocalization in live cell fluorescence microscopy
نویسندگان
چکیده
منابع مشابه
Colocalization analysis in fluorescence microscopy.
The measurement of colocalization requires images of two fluorophores that are aligned, with no cross talk, and that the intensities remain within the response range of the microscope. Quantitation depends upon differentiating between the presence and absence of fluorescence, and measurements should be made within biologically relevant regions of interest. Co-occurrence can be measured simply b...
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ژورنال
عنوان ژورنال: Journal of Biophotonics
سال: 2013
ISSN: 1864-063X
DOI: 10.1002/jbio.201300146